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KMID : 1007520020110020105
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Food Science and Biotechnology
2002 Volume.11 No. 2 p.105 ~ p.109
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Detection of Genetically Modified Glyphosate-Tolerant Soybeans
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Son Dae-Yeul
Lee Sang-Il
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Abstract
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The agricultural group of Monsanto Company has developed a new line of soybeans (Roundup Ready) by the addition of glyphosate-tolerant gene from Agrobacterium. This genetically modified (GM) soybean line carries the added gene and expresses 5-enol-pyruvyl-shikimate-3phosphate synthase (EPSPS) and is tolerant to glyphosate, the active ingredient from the world widely used herbicide Roundup. In this paper we report a new developed method for identifying the Roundup Ready soybeans. The method is based on the use of a nested polymerase chain reaction to determine the cauliflower mosaic virus 35S promoter, chloroplast transit peptide and EPSPS gene from Agrobacterium. GM soybean DNA extracted from a standard sample containing 0.1% GM soybean could be detected correctly. The method was applied to samples of soybean powder, soybean sprouts and processed products obtained from the markets in Korea. GM soybean DNA could be detected even in processed products such as soybean curd. This GM soy-specific detection method can be used as a sensitive tool for the screening of the presence of Roundup Ready soybeans in processed products made of soybeans.
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